Identification and determination of biocides by UPLC-MS / MS

Isothiazolinones, belonging to the biocides family, are frequently used in cosmetic products and detergents as preservatives and antimicrobial agents. They can also enter the process of making paper for food packaging to inhibit bacterial growth.

These molecules being toxic in aqueous medium, irritating and strongly allergenic on the cutaneous level, their use is framed by the texts of European laws [1, 2, 3].


To ensure the conformity of your products, an analysis by liquid chromatography coupled with tandem mass spectrometry is possible by Polymex.
Our UPLC-MS/MS device allows their identification and quantification with an LOQ of the order of 10 µg/L.

Identification et dosage de biocides

1. Regulation (EC) No 1223/2009 of the European parliament and of the council of 30 November 2009 on cosmetic products
2. Regulation (EC) No 648/2004 of the European parliament and of the council of 31 March 2004 on detergents
3. Regulation (EC) No 528/2012 of the European parliament and of the council of 22 May 2012 concerning the making available on the market and use of biocidal products


Polymex performs for you analyzes in different matrices related to the numerous professional sectors concerned by the presence of isothiazolinones:

- Agriculture (Use in pesticides, insecticides),
          - Metallurgy and mechanics (Use in cutting oils, coolants, lubricants, kerosene),
          - Construction and public works (Use in water-based paints, lacquers, latex emulsions, glues and adhesives, cooling water),
          - Hygiene (Use in soaps, disinfectant and detergents),
          - Cosmetics (Use in shampoos, hair lotions and gels, creams, cleansing wipes).
          (...)

Dosage d'isothiazolinones

You are looking to dose isothiazolinones in any type of matrix, for example:

  • Methylisothiazolinone = MIT [2682-20-4]
  • Methylchloroisothiazolinone = MCI [26172-55-4]
  • Benzisothiazolinone = BIT [2634-33-5]
  • Octylisothiazolinone = ILO [26530-20-1]
  • Butylbenzisothiazolinone = BBIT [4299-07-4]
  • Dichlorooctylisothiazolinone = DCOIT [64359-81-5]

Identification and determination of biocides

More info

Identification and dosages of phthalates

More info

Determination of nitrosamines

More info

Determination of acrylamide

More info